Hybridization Capture and PCR Amplification: Compa..

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Hybridization Capture and PCR Amplification: Comparing Two Key Enrichment Strategies in NGS | #amplification-Based Target Enrichment

Hybridization Capture and PCR Amplification: Comparing Two Key Enrichment Strategies in NGS

Hybridization Capture and PCR Amplification: Comparing Two Key Enrichment Strategies in NGS

In a hybridization assay, the protocols start with random shearing of the genes, denatured by heating. Randomly sheared overlapping fragments are captured by DNA or RNA single-stranded oligonucleotides specific to the region of interest. DNA bound to the baits is separated from background

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